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cll cell line jvm3  (DSMZ)


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    DSMZ cll cell line jvm3
    Fig. 4 U2-2P is incorporated in the spliceosome and post-transcriptionally modified. a RT-PCR of U2 and U2-2P with specific primers for each copy or with common primers for both on total extracts from <t>CLL</t> <t>JVM3</t> cells, or in immunoprecipitated material with anti-SAP155 antibody or with an isotype IgG. In the lower panel, control western blot for the detection of SAP155 in total cell extracts, immunoprecipated with control IgG or with anti-SAP155 antibody from the same experiment as above. b Pseudouridine mapping using U2- or U2-2P-specific primers showing similar pseudouridylation patterns in both snRNAs. On top, electropherogram corresponding to the Sanger sequence of U2/ U2-2P with the same primer used for the pseudouridylation analysis.
    Cll Cell Line Jvm3, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cll cell line jvm3/product/DSMZ
    Average 94 stars, based on 50 article reviews
    cll cell line jvm3 - by Bioz Stars, 2026-06
    94/100 stars

    Images

    1) Product Images from "PanCancer analysis of somatic mutations in repetitive regions reveals recurrent mutations in snRNA U2."

    Article Title: PanCancer analysis of somatic mutations in repetitive regions reveals recurrent mutations in snRNA U2.

    Journal: NPJ genomic medicine

    doi: 10.1038/s41525-022-00292-2

    Fig. 4 U2-2P is incorporated in the spliceosome and post-transcriptionally modified. a RT-PCR of U2 and U2-2P with specific primers for each copy or with common primers for both on total extracts from CLL JVM3 cells, or in immunoprecipitated material with anti-SAP155 antibody or with an isotype IgG. In the lower panel, control western blot for the detection of SAP155 in total cell extracts, immunoprecipated with control IgG or with anti-SAP155 antibody from the same experiment as above. b Pseudouridine mapping using U2- or U2-2P-specific primers showing similar pseudouridylation patterns in both snRNAs. On top, electropherogram corresponding to the Sanger sequence of U2/ U2-2P with the same primer used for the pseudouridylation analysis.
    Figure Legend Snippet: Fig. 4 U2-2P is incorporated in the spliceosome and post-transcriptionally modified. a RT-PCR of U2 and U2-2P with specific primers for each copy or with common primers for both on total extracts from CLL JVM3 cells, or in immunoprecipitated material with anti-SAP155 antibody or with an isotype IgG. In the lower panel, control western blot for the detection of SAP155 in total cell extracts, immunoprecipated with control IgG or with anti-SAP155 antibody from the same experiment as above. b Pseudouridine mapping using U2- or U2-2P-specific primers showing similar pseudouridylation patterns in both snRNAs. On top, electropherogram corresponding to the Sanger sequence of U2/ U2-2P with the same primer used for the pseudouridylation analysis.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation, Control, Western Blot, Sequencing



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    Fig. 4 U2-2P is incorporated in the spliceosome and post-transcriptionally modified. a RT-PCR of U2 and U2-2P with specific primers for each copy or with common primers for both on total extracts from <t>CLL</t> <t>JVM3</t> cells, or in immunoprecipitated material with anti-SAP155 antibody or with an isotype IgG. In the lower panel, control western blot for the detection of SAP155 in total cell extracts, immunoprecipated with control IgG or with anti-SAP155 antibody from the same experiment as above. b Pseudouridine mapping using U2- or U2-2P-specific primers showing similar pseudouridylation patterns in both snRNAs. On top, electropherogram corresponding to the Sanger sequence of U2/ U2-2P with the same primer used for the pseudouridylation analysis.
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    Fig. 4 U2-2P is incorporated in the spliceosome and post-transcriptionally modified. a RT-PCR of U2 and U2-2P with specific primers for each copy or with common primers for both on total extracts from <t>CLL</t> <t>JVM3</t> cells, or in immunoprecipitated material with anti-SAP155 antibody or with an isotype IgG. In the lower panel, control western blot for the detection of SAP155 in total cell extracts, immunoprecipated with control IgG or with anti-SAP155 antibody from the same experiment as above. b Pseudouridine mapping using U2- or U2-2P-specific primers showing similar pseudouridylation patterns in both snRNAs. On top, electropherogram corresponding to the Sanger sequence of U2/ U2-2P with the same primer used for the pseudouridylation analysis.
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    cll derived cell lines jvm3  (DSMZ)
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    Fig. 4 U2-2P is incorporated in the spliceosome and post-transcriptionally modified. a RT-PCR of U2 and U2-2P with specific primers for each copy or with common primers for both on total extracts from <t>CLL</t> <t>JVM3</t> cells, or in immunoprecipitated material with anti-SAP155 antibody or with an isotype IgG. In the lower panel, control western blot for the detection of SAP155 in total cell extracts, immunoprecipated with control IgG or with anti-SAP155 antibody from the same experiment as above. b Pseudouridine mapping using U2- or U2-2P-specific primers showing similar pseudouridylation patterns in both snRNAs. On top, electropherogram corresponding to the Sanger sequence of U2/ U2-2P with the same primer used for the pseudouridylation analysis.
    Cll Derived Cell Lines Jvm3, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cll derived cell lines jvm3/product/DSMZ
    Average 94 stars, based on 1 article reviews
    cll derived cell lines jvm3 - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 4 U2-2P is incorporated in the spliceosome and post-transcriptionally modified. a RT-PCR of U2 and U2-2P with specific primers for each copy or with common primers for both on total extracts from CLL JVM3 cells, or in immunoprecipitated material with anti-SAP155 antibody or with an isotype IgG. In the lower panel, control western blot for the detection of SAP155 in total cell extracts, immunoprecipated with control IgG or with anti-SAP155 antibody from the same experiment as above. b Pseudouridine mapping using U2- or U2-2P-specific primers showing similar pseudouridylation patterns in both snRNAs. On top, electropherogram corresponding to the Sanger sequence of U2/ U2-2P with the same primer used for the pseudouridylation analysis.

    Journal: NPJ genomic medicine

    Article Title: PanCancer analysis of somatic mutations in repetitive regions reveals recurrent mutations in snRNA U2.

    doi: 10.1038/s41525-022-00292-2

    Figure Lengend Snippet: Fig. 4 U2-2P is incorporated in the spliceosome and post-transcriptionally modified. a RT-PCR of U2 and U2-2P with specific primers for each copy or with common primers for both on total extracts from CLL JVM3 cells, or in immunoprecipitated material with anti-SAP155 antibody or with an isotype IgG. In the lower panel, control western blot for the detection of SAP155 in total cell extracts, immunoprecipated with control IgG or with anti-SAP155 antibody from the same experiment as above. b Pseudouridine mapping using U2- or U2-2P-specific primers showing similar pseudouridylation patterns in both snRNAs. On top, electropherogram corresponding to the Sanger sequence of U2/ U2-2P with the same primer used for the pseudouridylation analysis.

    Article Snippet: CLL cell line JVM3 (DSMZ) was grown in RPMI 1640, 10% FBS, 1% PSG and 1% non-essential amino acids.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation, Control, Western Blot, Sequencing